Transgenesis platform

This platform offers the possibility to generate genetically modified mouse models at low cost. The service includes conventional microinjection, either the random insertion of a gene, or targeted modifications with the CRISPR / Cas9 method. In addition, complementary services such as the cryopreservation of lineage (sperm or embryos) and the regeneration of lineage by IVF (from sperm or frozen embryos) are available.

Information request

Services :

  • Production of transgenic mice
  • Sperm or embryos cryopreservation
  • IVF for in vitro validation or restoration of lines
  • Reimplantation

Production of transgenic mice

Microinjection of 150-200 embryos per session. The surviving embryos are transferred to pseudopregnant females. The transgenesis platform of UQAM guarantees at least 2 transgenic founder mice (regardless of transgene expression).

 

Sperm or embryos cryopreservation

Cryopreservation of lines is done by freezing sperm or embryos.
For optimal sperm cryopreservation, males aged between 6 weeks and 6 months, and having produced at least one litter are recommended. Two males of the same line usually give 10 frozen sperm straws.
The embryo cryopreservation service will be available in spring 2019. Contact us for more information.

 

IVF for in vitro validation or restauration of lines

Validation method of the capacity of frozen sperm to fertilize. Motility of spermatozoa after thawing and the presence of stage 2 embryos are evaluated.
It is possible to go further with the reimplantation of embryos

 

Reimplantation

Surgical reimplantation in pseudopregnant females of embryos resulting from IVF or thawing

Price :

 Prices***
Microinjection
(2 sessions)
6000$
Microinjection CRISPR**
(1 session)
6000$
Sperm cryopreservation255$
Embryo cryopreservation**750$
IVF validation on 2 cells
(without sperm cryopreservation)
750$
IVF in vivo validation
(without sperm cryopreservation)
1460$
strain recovery from IVF2250$
Embryo implantation1200$

* Fees apply for insertion of any DNA sequence
** Price for a group of 5 donor females
*** Could change according to the particularities of each project

Additional 15% administration fees are required

Preferential prices for academic researchers

 Prices***
Microinjection
(2 sessions)
3000$
Microinjection CRISPR**
(1 session)
3000$
Sperm cryopreservation130$
Embryo cryopreservation**380$
IVF validation on 2 cells
(without sperm cryopreservation)
380$
IVF in vivo validation
(without sperm cryopreservation)
730$
strain recovery from IVF1200$
Embryo implantation600$

* Fees apply for insertion of any DNA sequence
** Price for a group of 5 donor females
*** Could change according to the particularities of each project

Researchers from the Universités du Québec network and members of CERMO-FC are invited to contact us for privileged prices thanks to the support of CERMO-FC