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Consulting and project development

We offer a personalized project development and consultation service
Please contact us for more information.

Cellular and enzymatic assays

TECAN M1000 Plate Reader

Various cellular and enzymatic assays, including AlphaScreen and AlphaLISA technologies

  • Absorbance
  • AlphaScreen
  • AlphaLISA Luminescence
  • TR-FRET
  • BRET 1-2-3
  • Fluorescence polarization
  • Fluorescence from above and below

Price

CERMO-FC members and UQAM researchers are invited to contact us to find out about privileged rates thanks to the support of CERMO-FC.

$15 /hr for stand-alone use or $30 /hr with technical assistance *.
* excluding the cost of consumables.

$40 /hr for stand-alone use or $50 /hr with technical assistance *.

* excluding the cost of consumables, and administrative charge of 15% may be applicable.

Characterizations of molecular interactions

Surface Plasmon Resonance (SPR) – Biacore T200

The versatile surface plasmon resonance apparatus allows the study of biomolecular interactions without protein modification; 1/ characterization of protein-protein, protein-DNA / RNA, protein-ligand interactions, 2/ Determination of kinetic binding parameters (kon, koff), 3/ determination of thermodynamic binding parameters (KD, ΔG, ΔH, ΔS). Precise and high sensitivity analyzes (results analysis software: Biacore T200 Evaluation).

Detection chips allowing immobilization modes:

  • Covalent: amines, thiols, aldehydes
  • Capture: biotin, His-tag
  • Hydrophobic: single and double layer
  • Possibility of custom attachment method

Circular dichroism spectrometer – JASCO J-815

This circular dichroism spectrometer enables rapid, simple, and non-destructive analysis of protein, DNA/RNA structures, their conformational transition induced by interactions, and their thermodynamic stability.

Multiple analysis mode:

  • wavelength scan (163 to 900 nm)
  • time scan
  • temperature sweep (-10 to 110 ° C)

Special feature of the device:

  • with Stopped-Flow system SFS-490
  • Results analysis software (Jasco SpectraManager)
  • Secondary structure analysis
  • Protein denaturation analysis
  • Curve smoothing analysis

Price

CERMO-FC members and UQAM researchers are invited to contact us to find out about privileged rates thanks to the support of CERMO-FC.

Biacore T200: $30 /hr for use and $30 /hr for technical assistance *.
JASCO J-815: $20 /hr in stand-alone use or $30 /hr with technical assistance.

* excluding the cost of microchips

Biacore T200: 110$ /h of use and 50$ /h of technical assistance*.
JASCO J-815: $40 /hr of use or $50 /hr with technical assistance.

*excluding the cost of microchips

An administrative charge of 15% may be applicable.

Purification of small molecules and peptides

Purification system – Waters

The Waters automatic purification system offers the specificity of directed purification through the inclusiveness of a UV system. With this state-of-the-art system, it is possible to purify small molecules and peptides.

  • Purification capacity up to 200 mg of sample
  • Reverse phase purification (RP-HPLC) by C4 or C18 column
  • Direct analysis by analytical HPLC from certain samples

Price

CERMO-FC members and UQAM researchers are invited to contact us to find out about privileged rates thanks to the support of CERMO-FC.

$50 /hr with technical assistance *.
* excluding the cost of consumables.

$100 /hr with technical assistance*.

*excluding the cost of microchips, and administrative charge of 15% may be applicable.

Synthesis of peptides

Synthesis of peptides, unnatural peptides, including fluorescent analogs, isotopically labeled peptides (15N, 13C) and post-translational modifications, such as phosphorylation and palmitylation

Synthesis of peptide of 8 to 50 residues with purification by RP-HPLC (purity >98% guaranteed), and characterization by mass spectrometry (ESI-TOF). Production of 10mg.

Price

CERMO-FC members and UQAM researchers are invited to contact us to find out about privileged rates thanks to the support of CERMO-FC.

  • Synthesis with purity >98%, 10mg:
  • 8-30 residue peptide: $27 /amino acid*
  • 31-40 residue peptide: $32 /amino acid*
  • 41-50 residue peptide: $37 /amino acid*

*For synthesis with special amino acids (N15, C13, unnatural) and other modifications (fluorophores, pegylation, phosphorylation), please contact us for a quote.

Synthesis with purity >98%, 10mg:
8-30 residue peptide: $35 /amino acid*
31-40 residue peptide: $40 /amino acid*
41-50 residue peptide: $45 /amino acid*

*For synthesis with special amino acids (N15, C13, unnatural) and other modifications (fluorophores, pegylation, phosphorylation), please contact us for a quote.

An administrative charge of 15% may be applicable.

Recommendations

We used the plate reader on this platform, which is very easy to book and use. The technical staff are also very pleasant and professional.

FAQ - Frequently Ask Questions

Fill out the “Service Request” form at the top of this page or contact us.

Contact us for a free consultation. Together we will assess the feasibility of your project, and we will discuss how to achieve your goals. Then, we will discuss the steps needed to start your project.

The analysis time of your data depends on your needs. The order of service requests is based on the date samples are received.

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